Gano Nucleic Acid Farin Tube
Takaitaccen Bayani:
Ana amfani da shi musamman don gano acid nucleic, kuma an samar da shi gabaɗaya a ƙarƙashin yanayin tsarkakewa, wanda ke rage yuwuwar gurɓataccen gurɓataccen abu yayin aiwatar da samarwa kuma yadda ya kamata ya rage tasirin yuwuwar gurɓatawa a kan gwaje-gwaje.
1. Gwajin ƙarar tsotsa: Ƙarar tsotsa, wato, adadin jinin da aka zana, yana da kuskure a cikin ± 10%, in ba haka ba bai cancanta ba.Rashin daidaiton adadin jinin da aka zana babbar matsala ce a halin yanzu.Wannan ba kawai yana haifar da sakamakon binciken da ba daidai ba, amma yana haifar da toshewa da lalata kayan aikin dubawa.
2. Gwajin yabo kwantena: Tushen tarin jini mai ɗauke da sinadarin sodium fluorescein an sanya shi a kife cikin ruwan da aka ƙera na tsawon mintuna 60.A ƙarƙashin tushen hasken ultraviolet mai tsayi mai tsayi, ba a sami haske ba a ƙarƙashin hangen nesa na yau da kullun a cikin ɗakin duhu, wanda ya cancanta.Zubewar kwantena shine babban dalilin rashin daidaitaccen adadin jini na bututun tarin jini na yanzu.
3. Gwajin ƙarfin kwantena: an sanya kwantena a cikin centrifuge tare da haɓakar centrifugal na 3000g na minti 10, kuma yana da cancanta idan bai fashe ba.Abubuwan da ake buƙata a ƙasashen waje sune: mita 2 sama da ƙasa, bututun tattara jini ya faɗi a tsaye ba tare da karye ba, wanda zai iya hana lalacewar bututun gwaji na bazata da asarar samfurori.
4. Mafi ƙarancin gwajin sararin samaniya: Mafi ƙarancin sarari don tabbatar da cewa jinin ya haɗu sosai.Adadin jinin da aka zana shine 0.5ml-5ml,>+25% na adadin jinin da aka zana;idan adadin jinin da aka zana ya kasance> 5ml,>15% na adadin jinin da aka zana.
5. Daidaitaccen gwaji na ƙaura, rabo mai yalwaci da adadin ƙarin bayani: kuskuren ya kamata ya kasance a cikin ± 10% na ƙayyadadden ma'auni na shuka.Wannan matsala ce da ba a iya mantawa da ita cikin sauki, kuma tana daya daga cikin manyan dalilan rashin ingancin bayanan gwaji.
