Tube Rarraba Gel guda ɗaya na Muclear Cell - Tube CPT

Bututun Rabewar Gel guda ɗaya na Muclear-CPT Tube Featured Image

Takaitaccen Bayani:

Ana amfani dashi don ware monocytes daga jini gaba ɗaya.

An fi amfani dashi don gano aikin rigakafi na lymphocyte kamar HLA, gano cutar sankarar bargo na saura da kuma maganin ƙwayoyin cuta.

Aika mana imel

Cikakken Bayani

Tags samfurin

Menene CPT Tube

Single muclear cell gel seperation tube (CPT tube) an ƙara da hypaque, anticoagulant da rabuwa gel.Lymphocytes da sel mononuclear za a iya keɓanta cikin sauƙi daga jini gaba ɗaya ta hanyar ɗamarar mataki ɗaya lokacin amfani da gel na rabuwar tantanin halitta na musamman.An fi amfani dashi don gano aikin rigakafi na lymphocyte, HLA ko ragowar cutar sankarar bargo da kuma maganin rigakafi.Yana ba da madaidaicin hanya don cirewar mataki ɗaya na monocytes don shirye-shiryen gwaji na asibiti da rigakafi na salula.

Ayyukan samfur

1) Girman: 13 * 100mm, 16 * 125mm;

2) Ƙara Ƙarar: 0.1ml, 135usp;

3) Girman Jini: 4ml,8ml;

4) Rayuwar Rayuwa: watanni 24 daga ranar da aka yi;

5) Adana:Adana a 18-25℃.

SamfuraAmfani

1) Ingantacce, daidai kuma mai aminci;

2) Tare da ginanniyar Ficoll Hypaque, anticoagulant da gel rabuwa, ƙwayoyin mononuclear sun rabu da jini duka ta hanyar centrifugation mataki ɗaya.

3) Madaidaicin fasahar rabuwar tantanin halitta.

4) Fasahar sarrafa membrane bionic bangon ciki;

5) Yawan dawo da monocytes ya fi 90%, tsarki ya fi 95%, kuma adadin rayuwa ya fi 99%

Abubuwan Bukatar Kulawa

Ya kamata a lura da waɗannan abubuwan:

1) A lokacin da yin gwaji na culturing Kwayoyin, kula da aseptic aiki, bakara da reagents (rarrabuwa bayani, wanka bayani, da dai sauransu) da kuma kayan aiki .Wannan aiki ya kamata a yi aiki da ƙwararru don tabbatar da haɗin kai na aiki.

2) Centrifugation zafin jiki ne kullum a dakin zafin jiki (2 ~ 25 ℃).

3) Yawancin lokaci lokacin da ke raba ƙwayoyin mononuclear (PBMC) tare da Ficoll, adadin jan jini yana da ƙananan, wanda zai iya rinjayar daidaiton gwajin.Don haka ana iya amfani da lysate (wasu suna buƙatar haifuwa), sarrafa lokacin lysis kuma hana yin tasiri akan ayyukan sel mononuclear.

4) Yi hankali game da sake-centrifugation wanda zai iya ninka dilution.

Na baya: CTAD Gane Tube

Na gaba: Bututun Jinin Jini