Platelet-rich Plasma (PRP) shine ma'auni mai sarrafa kansa na ɗan adam a cikin plasma.Ta hanyar lalata alpha granules a cikin platelets, PRP na iya ɓoye abubuwan haɓaka daban-daban, ciki har da nau'in haɓakar haɓakar platelet (PDGF), Factor endothelial girma factor (VEGF), fibroblast girma factor (FGF), hepatocyte girma factor (HGF), da kuma canzawa. ma'aunin girma (TGF), wanda aka rubuta don fara warkar da raunuka da kuma inganta haɓakawa da kuma canzawa na sel na endothelial da pericytes zuwa sprouts na endothelial.
An ba da rahoton ayyukan PRP don maganin ci gaban gashi a yawancin bincike na baya-bayan nan.Uebel et al.sun gano cewa abubuwan haɓakar jini na platelet suna ƙara yawan amfanin raka'o'in follicular a cikin tiyatar gashin gashi na namiji.Ayyukan da aka yi kwanan nan sun nuna cewa PRP yana ƙara haɓakar ƙwayoyin papilla dermal da kuma haifar da saurin telogen-to-anagen ta amfani da in vivo da in vitro model.Wani bincike ya nuna cewa PRP na inganta gyaran gashin gashi kuma yana rage yawan lokacin samuwar gashi.
Dukansu PRP da platelet-poor plasma (PPP) sun haɗa da cikakken haɗin sunadaran coagulation.A cikin binciken yanzu, an bincika tasirin PRP da PPP akan haɓakar gashi a cikin mice C57BL / 6.Ma'anar ita ce PRP yana da tasiri mai kyau a kan tsayin gashi da kuma karuwar adadin gashin gashi.
Dabbobin gwaji
Gabaɗaya 50 lafiya C57BL / 6 mice maza (makonni 6, 20 ± 2 g) an samo su daga Cibiyar Dabbobi na Laboratory, Jami'ar Al'ada ta Hangzhou (Hangzhou, China).An ciyar da dabbobi abinci iri ɗaya kuma ana kiyaye su a cikin yanayi na yau da kullun ƙarƙashin yanayin duhu-12:12-h.Bayan mako 1 na haɓakawa, an raba mice zuwa ƙungiyoyi uku: ƙungiyar PRP (n = 10), ƙungiyar PPP (n = 10), da ƙungiyar kulawa (n = 10).
Kwamitin da'a na hukumomi na binciken dabbobi ya amince da ka'idar binciken karkashin dokar binciken dabbobi da ka'idojin doka a kasar Sin.
Tsawon gashi
A kwanaki 8, 13, da 18 bayan allura ta ƙarshe, gashi 10 a cikin kowane linzamin kwamfuta an zaɓi bazuwar a yankin da aka yi niyya.An gudanar da ma'aunin tsawon gashi a wurare uku ta hanyar amfani da na'urar duban dan adam, kuma an bayyana matsakaicin su a matsayin millimeters.An cire gashin da aka yi wa tsayi ko lalacewa.
Hematoxylin da eosin (HE) tabo
An cire samfuran fata na dorsal a kwanaki 18 bayan allurar ta uku.Sa'an nan kuma an gyara samfurori a cikin 10% tsaka tsaki buffered formalin, saka a cikin paraffin, kuma a yanka a cikin 4 μm.An gasa sassan na tsawon sa'o'i 4 don deparaffinization a 65 ° C, a tsoma su cikin ethanol gradient, sa'an nan kuma an lalata su da hematoxylin na 5 min.Bayan da aka bambanta a cikin 1% hydrochloric acid barasa, an sanya sassan a cikin ruwan ammonia, an lalata su da eosin, kuma an wanke su da ruwa mai tsabta.A ƙarshe, sassan sun bushe tare da gradient ethanol, an share su tare da xylene, an saka shi da resin tsaka tsaki, kuma an lura da su ta amfani da ƙaramin haske (Olympus, Tokyo, Japan).
Lokacin aikawa: Oktoba-12-2022