Gel Yellow Tarin Jini
Takaitaccen Bayani:
Don gano sinadarai, gwaje-gwajen rigakafi, da sauransu, ba a ba da shawarar tantance abubuwan ganowa ba.
Fasahar zafin jiki mai tsafta tana tabbatar da ingancin sinadirai, ƙarancin ajiyar zafin jiki, da daskararre na samfuran yana yiwuwa.
An yi amfani da bututun tattara jini na Gel a cikin dakunan gwaje-gwaje na asibiti.Rarraba gel na iya samar da keɓancewar Layer tsakanin sassan sel da jini (plasma), yadda ya kamata ya hana musayar abu tsakanin ƙwayoyin jini da jini (plasma), da tabbatar da daidaiton abubuwan serum (plasma) a cikin wani ɗan lokaci.Rarraba manne ne yafi hada da silicone roba, macromolecular hydrocarbons, hydrophobic manne, da dai sauransu A matsayin polymer abu, shi ne insoluble a ruwa da inert.Ruwa ne mai danko thixotropic tare da yawa na 1.04-1.05 mmol / Tsakanin L, yana da fa'idodin juriya na iskar shaka, juriya mai zafin jiki, ƙarancin zafin jiki, da ƙarancin iska mai kyau.Matsakaicin adadin jini shine 1.026-1.031 mmol/L, kuma hematocrit shine 1.090-1.095.Saboda ƙayyadaddun nauyin nauyi, rabuwar gel ɗin yana tsakanin kwayoyin jini da jini, don haka a karkashin yanayi na al'ada, jini zai bayyana a jere bayan centrifugation.Serum, separating gel, da jini Kwayoyin 3 benaye.
Gabaɗaya nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan da ake amfani da su ana amfani da su a dakunan gwaje-gwaje ne: bututun rabuwa da gel procoagulation tube da bututun rabuwa da gel anticoagulation tube.Maganin rabuwar gel procoagulation tube shine ƙara coagulant a cikin bututun tattara jini don rage lokacin coagulation na jini, samun ruwan magani cikin sauri, da bayar da rahoton sakamakon a cikin mafi ƙanƙan lokaci.Gilashin tarin jini ba sa buƙatar ƙara masu coagulant, kuma jinin tuntuɓar bangon bututun gilashi zai haifar da coagulation.Koyaya, lokacin da abubuwan coagulation XI da XII suka haɗu da bututun tattara jini na filastik, ikon kunna su yana da rauni sosai, kuma ana buƙatar ƙara coagulant don rage lokacin coagulation.Ana fesa bututun rigakafin ƙwayar cuta ta plasma tare da maganin rigakafi kamar lithium heparin akan bangon ciki na bututun tattara jini na gel don saduwa da buƙatun gwajin gaggawa na biochemical na plasma cikin sauri.
A cikin aikace-aikacen aikace-aikacen, sau da yawa ana saduwa da cewa tasirin rabuwa na jigilar jigilar jigilar jigilar jini ba shi da kyau, alal misali: a cikin wasu bututun roba na rabuwa, ana iya ganin cewa gutsuttsuran gel ɗin rabuwa ko ɗigon mai suna iyo a saman saman. magani ko dakatarwa a cikin jini;Layer gel ɗin rabuwa yana yawo a kan layin ruwan magani.sama da sauransu. Rarrabe gels kuma na iya tsoma baki tare da wasu sakamakon gwaji.A cikin sashinmu, an gano cewa takamaiman tsari na reagents da bututun rabuwar jini na jini sun amsa da juna yayin binciken HBSAg na tsarin gano Abbott i2000SR, wanda ya haifar da sakamako mai kyau na ƙarya.
Wannan takarda ya fi yin nazari ne daga bangarori biyu, wato, dalilan da ke haifar da mummunan tasirin rabuwar gel din, da kuma tasirin gabatarwar gel mai rarraba akan ma'auni.
1. Hanya na rabuwa da jini da plasma ta hanyar raba gel Rarraba gel shine mucocolloid na thixotropic wanda ya hada da mahadi na hydrophobic da silica foda.Tsarin ya ƙunshi adadi mai yawa na haɗin hydrogen.Kasancewar haɗin gwiwar hydrogen ya ƙunshi tushen sinadarai na thixotropy na gel mai raba..Ana kiyaye ƙayyadaddun nauyi na gel ɗin rabuwa a 1.05, ƙayyadaddun nau'in nau'in ruwa na jini shine kusan 1.02, kuma takamaiman nau'in nau'in nau'in jini yana kusan 1.08.Lokacin da keɓaɓɓen gel da jinin da aka haɗa (ko anticoagulated duka jini) suna centrifuged a cikin bututun gwajin guda ɗaya, Saboda ƙarfin centrifugal da aka yi amfani da gel ɗin raba, tsarin cibiyar sadarwar hydrogen bond ya karye cikin tsari mai kama da sarkar, da rabuwar. gel ya zama ruwa mai ƙarancin danko.Saboda nau'i na musamman daban-daban, gel ɗin rabuwa yana juyawa kuma an daidaita shi don samar da nau'i uku na jini na jini (anticoagulated dukan jini) / raba gel / serum (plasma).Lokacin da centrifuge ya daina juyawa kuma ya rasa ƙarfin centrifugal, sassan sarkar a cikin gel ɗin da aka raba su sake yin tsarin hanyar sadarwa ta hanyar haɗin gwiwar hydrogen, suna mayar da yanayin gel na farko mai danko, kuma su samar da keɓaɓɓen Layer tsakanin serum (plasma) da jini (anticoagulated). dukkan jini)..
2. Dalilai don mummunan tasirin rabuwa na rabuwar gel
2.1 Rarraba ingancin Gel Ƙaƙƙarfan nauyin nau'in gel ɗin yana tsakanin na jini (plasma) da ƙwayoyin jini, wanda shine tushen jiki don sake jujjuyawar gel ɗin da kuma rabuwar jini (plasma).Idan ingancin gel ɗin rabuwa na bututun jini bai da kyau kuma takamaiman nauyin nauyi bai cika buƙatun ba, to babu makawa zai yi tasiri tasirin rabuwar jini (plasma), da sabon abu cewa gel ɗin rabuwa da jini (plasma). mai yuwuwa a haɗa juna.
2.2 Rashin coagulation na jini wanda bai cika ba Bayan centrifugation, wani lokaci sashin gel na rabuwa da jini da ɗigon jini ba su rabu gaba ɗaya ba, kuma fibrin filaments suna bayyana a cikin maganin.Dalilin shi ne sau da yawa cewa jinin bai cika cika ba kafin centrifugation.Rashin cikakkiyar coagulation na jini na iya haifar da haɗewar fibrin a cikin keɓewar.Dole ne a yi amfani da bututun raba ruwan magani daidai bisa ga umarnin, kuma ana iya shirya maganin ta hanyar centrifugation bayan jinin ya cika gaba ɗaya (yawanci, bututun filastik da ke ɗauke da coagulant yana buƙatar a sanya shi a tsaye na kusan mintuna 30, kuma jinin ya kasance a tsaye). bututu mai tarin ba tare da coagulant yana buƙatar a sanya shi tsaye na mintuna 60-90).samfurori masu inganci masu inganci.
2.3 Centrifugation zafin jiki na centrifugation zafin jiki yana da tasiri mai mahimmanci akan tasirin rabuwa da kwayar cutar gel tube.Maganin ya fito fili a cikin inert separating gel accelerated coagulation tube rabu da wani talakawa centrifuge a dakin zafin jiki, amma m beads na daban-daban masu girma dabam ya bayyana a cikin 15% zuwa 20% na samfurori.A gefe guda kuma, ba a sami ƙwanƙwasa mai mai a cikin maganin da aka raba da bututun gwajin da aka yi da ƙananan zafin jiki ba.Lokacin da zafin jiki ya wuce yawan zafin jiki da ake buƙata don gel ɗin rabuwa, gel ɗin inert zai narke a cikin maganin.Ba wai kawai zai toshewa da gurɓata samfurin samfurin allura da kofin amsawa na mai nazarin kwayoyin halitta ba, har ma yana da babban tasiri akan wasu sakamakon auna sinadarai.
